Reporter
Part:BBa_K362061:Design
Designed by: Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama Group: iGEM10_KIT-Kyoto (2010-10-25)
oxyR promoter - RBS - GFP - Term - Term
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 122
Illegal BglII site found at 569
Illegal BglII site found at 1034
Illegal BamHI site found at 799
Illegal BamHI site found at 1023 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1726
Design Notes
This composite part contains oxyR promoter(BBa_K362004),RBS(BBa_K121013),GFP(BBa_K121013) and 2 terminators on low copy vector(BBa_K121013). oxyR promoter has sensitibity to oxydative stress.
Source
oxyR promoter from E.coli genome